LIG2

Sigma

 

QuickLink™ DNA Ligation Kit

Description

Features and Benefits• Fast 5 minute ligation.
• Perform at room temperature (does not require any cooling device).
• High ligation efficiency as detected by number of transformed colonies bearing a ligation product.
• Optimized for blunt- and sticky- ends ligation of restriction endonuclease digested inserts as well as PCR products.
• Bacterial transformation can be performed directly with the reaction mixture.
• Suitable for cloning into plasmids as well as phages, addition of linker (adapter), recircularization of linear DNA and concatamers formation.
Other NotesQuickLink has been optimized for efficient blunt and cohesive ligations performed at room temperature with a short incubation, replacing the previous methods requiring 16 °C and long incubations. It comes with pre-made buffers, depending on the buffer conditions of the DNA, for fast and easy set up times.
Legal InformationQuickLink is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

usage kit sufficient for 50 ligation reactions
shipped indry ice
storage temp.−70°C

Components

Standard componentT4 DNA ligase
 2X Ligation buffer A
 5X Ligation buffer B

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References

referenceGoncalves, P.P., et al., Ca2+-H+ antiport activity in synaptic vesicles isolated from sheep brain cortex. Neurosci. Lett. 247, 87-90, (1998) Abstract
 Lehman, I.R., DNA ligase: structure mechanism and function. Science 186, 790, (1974) Abstract
 Rossi, R., Functional characterization of the T4 DNA ligase: a new insight into the mechanism of action. Nucleic Acids Res. 25, 2106, (1997) Abstract
 Hayashi, K., et al., Regulation of inter- and intramolecular ligation with T4 DNA ligase in the presence of polyethylene glycol. Nucleic Acids Res. 14, 7617-7631, (1986) Abstract